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. 2017 Jun 5;97(1):30–37. doi: 10.4269/ajtmh.15-0771

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 2. — Spleen cell proliferation assay to measure cell-mediated immunization. The isolated spleen cells were restimulated with 1 μg of rec47. Cell viability was measured as the percentage of viable cells at 24, 48, and 72 hours after restimulation. Statistically significant differences between the PBS control group and each recombinant protein immunization group are indicated with * (P < 0.05) or ** (P < 0.01). Statistically significant differences between the pVAX1 control group and each DNA vaccination group are indicated with † (P < 0.05) or †† (P < 0.01).