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. 2017 Jun 5;97(1):30–37. doi: 10.4269/ajtmh.15-0771

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 3. — Cytokine release in immunized mice sera. After the third immunization, the concentration of distinct Th1- and Th2-type cytokines in mouse serum was measured by using a multiplex assay. (A) interferon gamma (IFN-γ), (B) interleukin (IL)-2, (C) IL-4, (D) IL-5, (E) IL-6, (F) IL-10, and (G) IL-12 (p70). Statistically significant differences between the PBS control group and each recombinant protein immunization group are indicated with * (P < 0.05) or ** (P < 0.01). Statistically significant differences between the pVAX1 control group and each DNA vaccination group are indicated with † (P < 0.05) or †† (P < 0.01).