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. 2017 Jun 5;97(1):30–37. doi: 10.4269/ajtmh.15-0771

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 4. — Cytokine release from spleen cells isolated from immunized mice. The isolated spleen cells were restimulated with 1 μg of rec47. Cytokines were measured at 24 hours after restimulation using an enzyme-linked immunosorbent assay. (A) interferon gamma (IFN-γ), (B) tumor necrosis factor (TNF)-α, (C) interleukin (IL)-2, (D) IL-5, (E) IL-6, and (F) IL-10. Statistically significant differences between the PBS control group and each recombinant protein immunization group are indicated with * (P < 0.05) or ** (P < 0.01). Statistically significant differences between the pVAX1 control group and each DNA vaccination group are indicated with † (P < 0.05) or †† (P < 0.01).