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. 2017 Jun 5;97(1):30–37. doi: 10.4269/ajtmh.15-0771

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© The American Society of Tropical Medicine and Hygiene

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Figure 5. — Evaluation of the protection against homologous challenge in immunized mice. Orientia tsutsugamushi strain Boryong was injected intraperitoneally into mice immunized with rec47 alone or rec47 plus LTB/CT and into mice immunized with p47 alone or p47 and pB, and their respective control treatments. The mortality was monitored daily for 2 weeks. The survival rate was calculated as the ratio of the number of living mice to the total number of challenged mice in a group. Statistically significant differences between the PBS control group and each recombinant protein immunization group are indicated with * (P < 0.05). Statistically significant differences between the pVAX1 control group and each DNA vaccination group are indicated with † (P < 0.05).