Figure 1.

Validation of markers on stage-specific in vitro culture materials: early ring-stage trophozoites, late ring-stage trophozoites, and stage V gametocytes. Total parasitemia was measured by targeting the (A) 18S DNA and (B) 18S RNA at the transcript level. (C) Gametocytemic signal was quantified by targeting Pfs25. (D–G) Transcription level of early ring-stage trophozoite markers were used to quantify ring-stage trophozoites. Presented is the dilution series of duplicate measurements of the Log₁₀ parasites/mL (x axis) and the corresponding CT values (y axis).