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Linear range of the ONNV real-time reverse transcription polymerase chain reaction performed in monoplex (black trend line) and as part of an ONNV–CHIKV duplex assay (gray trend line). Ct values for individual replicates are shown for the ONNV monoplex (•) and ONNV–CHIKV duplex (▴) assays. CHIKV = chikungunya virus; ONNV = O’nyong-nyong virus.
