Table 2.

Within-run, between-run, and total imprecision of the O’nyong-nyong virus (ONNV) real-time reverse transcription polymerase chain reaction determined using quantitated ssDNA controls

		Within-run		Between-run		Within-laboratory
Concentration*	Mean Ct	SD	%CV	SD	%CV	SD	%CV
8.0	12.06	0.31	2.54	0.23	1.92	0.32	2.63
6.0	19.00	0.45	2.36	0.17	0.89	0.36	1.89
4.0	25.60	0.89	3.48	0.24	0.92	0.67	2.63
2.0	35.25	1.06	2.99	0.83	2.35	1.12	3.17

%CV = percent coefficient variation; SD = standard deviation.

^*Values in log₁₀ copies/μL of eluate.