Fig. 6.

Effects of insulin signalling on cyclin D2 expression in beta cell lines. (a) Gene expression of cyclin D2 (Ccnd2) evaluated by quantitative real-time PCR in βIRKO mouse beta cells and control beta cells (LoxLoxβ). n=4 in each group. (b) Western blotting for cyclin D2 at indicated time periods after CHX treatment and graph showing quantification. Solid line, control LoxLoxβ cells; dashed line, βIRKO cells. (c) Western blotting for insulin receptor (IR) and cyclin D2 after infection of control cells with adenoviruses carrying GFP or Cre recombinase. Graphs show quantification. (d) Western blotting for IR and cyclin D2 after lentiviral gene transfer of IR to βIRKO cells. All protein expression was normalised to actin expression. All data are expressed as mean ± SEM. n=3 in each group. *p<0.05 vs control condition