Table.

Influence of the effectors on the activity of Lon-H₆ and Lon-dHI(CC) peptidase centers

Effector	Lon-H6		Lon-dHI(CC)
	v	n	v	n
No effector	5.88	1	1.64	1
Mg	33.1	5.62	5.19	3.16
ATP	47.1	8.01	1.33	0.81
ADP	0.49	0.08	1.79	1.09
AMPPNP*	14.2	2.41	1.82	1.11
ATP-Mg	63.5	10.8	4.62	2.82
ADP-Mg	10.1	1.73	4.89	2.98
AMPPNP-Mg	58.0	9.86	5.6	3.41

Note. The specific rates of PepTBE hydrolysis (v, ([S], μM)/([E], μM) min) are given; n is the ratio of substrate hydrolysis rates in the presence and absence of the effector (v_ef/v₀), where n < 1 corresponds to inhibition (italicized), and n > 1 corresponds to activation of hydrolysis (shown in bold). The error did not exceed 10%. Experimental conditions: 50 mM Tris-HCl buffer, pH 8.1; 0.15 M NaCl; 10% DMSO; 0.1 mM PepTBE; 0.2 mM DTDP; 2.5 mM Nu; 20 mM MgCl₂; 0.2 μM enzyme; 37°C.

^* Nonhydrolysable ATP analog, adenosine-5’-(β,γ-imido)triphosphate. ;