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. 2016 Sep 6;65(11):1377–1393. doi: 10.1007/s00262-016-1897-3

Table 2.

List of peptides identified from tumor-associated antigens

Gene name (protein name) Peptide sequence FM-82 FM-93/2 Mel-624 MeWo SK-Mel-5 HLA Alleles (IC50 in nM by NetMHCpan 2.8)
SEPT2 (septin-2) KVNIVPVIAK X A*03:01 (46 nM)
RLYPWGVVEV X X X A*02:01 (5 nM)
VEIEERGVKL X X B*40:01 (47 nM)
YIDEQFERY X A*01:01 (12 nM)
ALDH1A1 (retinal dehydrogenase 1) ASERGRLLY X A*01:01 (35 nM)
TMESMNGGKLY X No binding predicted
ARL4D (ADP-ribosylation factor-like protein 4D) RLYEMILKR X A*03:01 (51 nM)
ATIC (IMP cyclohydrolase) NLYPFVKTV X X A*02:01 (41 nM)
BCAP31 (B-cell receptor-associated protein 31) KLDVGNAEV X X X X X A*02:01 (27 nM)
CCNB1 (G2/mitotic-specific cyclin-B1) VMVNQGLTKHMTV X A*02:01 (93 nM)
VVMVNQGLTK X X A*11:01 (27 nM), A*03:01 (95 nM)
GEVDVEQHTL X X B*40:01 (10 nM)
TLAKYLMEL X X X X A*02:01 (7 nM)
CCND1 (G1/S-specific cyclin-D1) ALLESSLRQA X X X A*02:01 (90 nM)
EVFPLAMNY X X A*26:01 (22 nM)
EVFPLAMNYL X A*26:01 (221 nM), C*12:03 (176 nM)
CCNI (cyclin-I) AVLTKQLLH X No binding predicted
EVIQWLAKL X A*26:01 (11 nM)
LLDRFLATV X X X X A*02:01 (8 nM)
SLLDRFLATV X A*02:01 (4 nM)
CDR2 (cerebellar degeneration-related protein 2) NSELEQQLGATGAY X A*01:01 (67 nM)
RELEETNQKL X X B*40:01 (19 nM)
CEP55 (centrosomal protein of 55 kDa) AELESKTNTL X B*40:01 (34 nM)
EVHNLNQLLY X X A*26:01 (22 nM)
CPSF1 (cleavage and polyadenylation specificity factor subunit 1) ALDEKLLNI X A*02:01 (47 nM)
RLGNSLLLK X A*03:01 (17 nM)
TLITDGMRSV X X A*02:01 (76 nM)
VSDRDRNLMVY X A*01:01 (13 nM)
CSPG4 (chondroitin sulfate proteoglycan 4) ALTDIDLQL X A*02:01 (62 nM)
ASSSAGTDPQLLLY X A*01:01 (39 nM)
SSSAGTDPQLLLY X A*01:01 (14 nM)
STDPQHHAY X A*01:01 (5 nM)
TMLARLASA X X X A*02:01 (44 nM), B*08:01 (40 nM)
TPNPALKNGQYW X No binding predicted
DCT (l-dopachrome tautomerase) ELAPIGHNRMY X A*26:01 (50 nM)
ETHLSSKRY X X A*26:01 (207 nM)
GTYEGLLRR X X A*03:01 (421 nM), A*11:01 (56 nM)
KELPSLHVL X B*40:01 (18 nM)
LMETHLSSK X A*03:01 (122 nM)
MTVDSLVNK X A*11:01 (9 nM)
REQFLGALDL X X B*40:01 (13 nM)
SLDDYNHLV X A*02:01 (4 nM)
SLDDYNHLVTL X X X A*02:01 (10 nM)
TRPWSGPYIL X C*07:02 (182 nM)
TSDQLGYSY X A*01:01 (8 nM)
VEETPGWPTTL X X B*40:01 (61 nM)
YYSVRDTLL X C*07:02 (57 nM)
EHD2 (EH domain-containing protein 2) ALASHLIEA X X X A*02:01 (6 nM)
GQYSTGKTSFIQY X B*15:01 (40 nM)
KLPNSVLGR X No binding predicted
RVHAYIISY X A*03:01 (59 nM)
ENAH (protein enabled homolog) ERLERQERL X No binding predicted
GAGE12I (G antigen 12I) YYWPRPRRY X No binding predicted
GPR143 (G-protein-coupled receptor 143) ATSPPASVR X X A*11:01 (103 nM)
AVASLLKGR X No binding predicted
GLSTILLYH X A*03:01 (390 nM)
HAIPHYVTM X X X C*03:03 (5 nM), C*03:04 (5 nM)
MLYYPSVSR X A*03:01 (29 nM)
HMMR (hyaluronan mediated motility receptor) KLLEYIEEI X X A*02:01 (2 nM)
KLQEELNKV X X A*02:01 (12 nM)
RLNAALREK X A*03:01 (62 nM)
HMOX1 (heme oxygenase 1) EVIPYTPAM X X A*26:01 (5 nM)
EVIPYTPAMQR X No binding predicted
EVIPYTPAMQRY X A*26:01 (4 nM)
HNRNPL (heterogeneous nuclear ribonucleoprotein L) AAAGGGGGGGRY X X No binding predicted
AAAGGGGGGGRYY X B*15:01 (159 nM)
ETLGFLNHY X A*26:01 (14 nM)
KQPAIMPGQSY X B*15:01 (98 nM)
KSDALETLGFLNHY X No binding predicted
MAAAGGGGGGGRY X X X A*26:01 (170 nM), B*15:01 (59 nM)
YAVDRAITH X X C*12:03 (59 nM), C*03:04 (273 nM)
HSPA1L (heat-shock 70 kDa protein 1-like) LIFDLGGGTFD X No binding predicted
TVFDAKRLIGR X X A*11:01 (109 nM)
DTERLIGDAAKNQV X No binding predicted
RLIGDAAKNQV X X X X X A*02:01 (84 nM)
AMTKDNNLL X X No binding predicted
IGF2BP3 (insulin-like growth factor 2 mRNA-binding protein 3) EVLDSLLVQY X A*26:01 (47 nM)
KIQEILTQV X X X X A*02:01 (21 nM)
TLYNPERTITV X X A*02:01 (80 nM)
IL13RA2 (interleukin-13 receptor subunit alpha-2) LLDTNYNLF X No binding predicted
LLDTNYNLFY X A*01:01 (10 nM)
KDM5B (lysine-specific demethylase 5B) ALKDSVQRA X X No binding predicted
RLIDLGVGL X A*02:01 (20 nM)
RQFVTQLY X B*15:01 (30 nM)
SVAQQLLNGK X A*03:01 (20 nM)
KIF20A (kinesin-like protein KIF20A) KEAGNINTSL X B*40:01 (14 nM)
RVFQGFFTGR X X A*11:01 (22 nM), A*03:01 (72 nM)
SEHLDTQKELL X B*40:01 (80 nM)
LGALS3BP (galectin-3-binding protein) ALWKEPGSNV X A*02:01 (42 nM)
NLWDLTDASVV X A*02:01 (19 nM)
RIDITLSSV X A*02:01 (179 nM)
MAGEA1 (melanoma-associated antigen 1) ALREEEEGV X No binding predicted
EADPTGHSY X A*01:01 (79 nM)
EVYDGREHSA X No binding predicted
KEADPTGHSY X No binding predicted
KVLEYVIKV X A*02:01 (5 nM)
LTQDLVQEKYLEY X A*01:01 (42 nM)
SAYGEPRKL X C*03:04 (184 nM)
MAGEA12 (melanoma-associated antigen 12) EVVRIGHLY X A*26:01 (7 nM)
KMAELVHFL X X X A*02:01 (2 nM)
MAGEA12; MAGEA6; MAGEA3 (melanoma-associated antigen 12; 6; 3) KIWEELSVLE X No binding predicted
MAGEA2 (melanoma-associated antigen 2) EVFEGREDSVF X X A*26:01 (72 nM)
MAGEA2; MAGEA2B (melanoma-associated antigen 2; 2B) KMVELVHFL X A*02:01 (2 nM)
MAGEA1; MAGEA2; MAGEA2B; MAGEA3; MAGEA6 (melanoma-associated antigen 1; 2; 2B; 3; 6) REPVTKAEML X B*40:01 (255 nM)
MAGEA3 (melanoma-associated antigen 3) EVDPIGHLYIF X No binding predicted
MAGEA4 (melanoma-associated antigen 4) GVYDGREHTV X A*02:01 (225 nM)
MAGEA4; MAGEA8 (melanoma-associated antigen 4; 8) KVLEHVVRV X A*02:01 (9 nM)
MAGEA3; MAGE6 (melanoma-associated antigen 3; 6) KIWEELSVLEV X X A*02:01 (7 nM)
MAGEC2 (melanoma-associated antigen C2) ALIEVGPDHFC X No binding predicted
MDM2 (E3 ubiquitin–protein ligase Mdm2) RLYDEKQQHI X A*02:01 (72 nM)
RLYDEKQQHIVY X B*15:01 (54 nM)
MET (hepatocyte growth factor receptor) EVIGRGHFGCVY X A*26:01 (6 nM)
KSEMNVNMKY X A*01:01 (193 nM)
MFGE8 (lactadherin) EVRGDVFPSY X A*26:01 (15 nM)
MLANA (melanoma antigen recognized by T cells 1) AEEAAGIGIL X B*40:01 (9 nM)
ALMDKSLHV X A*02:01 (4 nM)
MMP2 (72 kDa type IV collagenase; PEX) AVVDLQGGGHSY X X A*26:01 (232 nM)
NELFA (negative elongation factor A) KLLDISELDMV X X X A*02:01 (4 nM)
NOB1 (RNA-binding protein NOB1) ALQDIGKNIYTI X A*02:01 (59 nM)
NPM1 (nucleophosmin) DIKAKMQAS X No binding predicted
FEITPPVVL X X B*40:01 (10 nM)
KMQASIEKA X X A*02:01 (472 nM)
RMTDQEAIQ X X No binding predicted
RMTDQEAIQDL X X X X A*02:01 (347 nM)
YEGSPIKVTL X X B*40:01 (24 nM)
NUDCD1 (NudC domain-containing protein 1) AEEHSIATL X B*40:01 (13 nM)
NUF2 (kinetochore protein Nuf2) ATAQFKINK X A*11:01 (11 nM)
EVVEKYEIY X A*26:01 (9 nM)
KIDEKTAELK X No binding predicted
OCA2 (P protein) HVDSTLLQ X No binding predicted
HVDSTLLQV X X X X A*02:01 (170 nM)
KEPSEIVEL X B*40:01 (104 nM)
PASD1 (PAS domain-containing protein 1) QLEERTWLL X A*02:01 (79 nM)
PGK1 (phosphoglycerate kinase 1) DNGAKSVVL X No binding predicted
EGKVLPGVDALSNI X No binding predicted
PLIN2 (perilipin-2) RAYQQALSR X A*03:01 (140 nM)
SLLTSSKGQLQK X A*03:01 (53 nM)
SVASTITGV X A*02:01 (55 nM)
TLLSNIQGV X A*02:01 (9 nM)
TSALPIIQK X A*11:01 (24 nM)
PMEL (melanocyte protein PMEL; M-alpha; M-beta) ALDGGNKHFL X X X X X A*02:01 (168 nM)
ALLAVGATK X A*03:01 (45 nM)
ALNFPGSQK X A*03:01 (21 nM)
AMLGTHTMEV X X X X X A*02:01 (8 nM)
AMLGTHTMEVTV X X A*02:01 (20 nM)
ASLIYRRRLMK X A*11:01 (61 nM)
FALQLHDPSGY X C*03:04 (250 nM)
FTITDQVPFSVSVSQLR X No binding predicted
GTATLRLVK X X A*11:01 (22 nM), A*03:01 (121 nM)
HQILKGGSGTY X B*15:01 (25 nM)
HSSSHWLRLP X X No binding predicted
HTYLEPGPVTAQ X No binding predicted
ITDQVPFSV X C*05:01 (37 nM), A*02:01 (188 nM)
ITDQVPFSVSVSQLR X No binding predicted
KQDFSVPQL X A*02:01 (455 nM)
KTWDQVPFS X No binding predicted
KTWDQVPFSV X X X X X A*02:01 (11 nM)
KTWDQVPFSVSV X A*02:01 (13 nM)
KTWGQYWQ X No binding predicted
KTWGQYWQV X X X X X A*02:01 (10 nM)
LSIGTGRAM X X C*03:03 (39 nM), C*03:04 (39 nM)
LVLKRCLLH X No binding predicted
MLGTHTMEV X X X X A*02:01 (8 nM)
RNQPLTFALQLHDPSGY X No binding predicted
RYGSFSVTL X C*07:02 (362 nM)
SLADTNSLAV X A*02:01 (13 nM)
SLADTNSLAVV X X X X X A*02:01 (12 nM)
SVPQLPHSSSHW X No binding predicted
TITDQVPFSVSVSQLR X No binding predicted
VLYRYGSFSV X X A*02:01 (6 nM)
VLYRYGSFSVTL X A*02:01 (41 nM)
YRYGSFSVTL X X C*07:01 (10 nM), C*03:03 126 (nM)
PPIB (peptidyl-prolyl cis–trans isomerase B) LLLPGPSAA X X X X A*02:01 (75 nM)
PRAME (MELANOMA antigen preferentially expressed in tumors) AAFDGRHSQTL X X X X C*03:03 (7 nM), C*03:04 (7 nM), C*12:03 (16 nM)
ALLERASATL X A*02:01 (25 nM)
ALLPSLSHC X X No binding predicted
GQHLHLETF X B*15:01 (96 nM)
QLLALLPSL X A*02:01 (9 nM)
RLDQLLRHV X X X X A*02:01 (217 nM)
RLVELAGQSLLK X A*03:01 (35 nM)
SLLQHLIGL X X A*02:01 (12 nM)
YEDIHGTLHL X B*40:01 (15 nM)
PRDX5 (peroxiredoxin-5, mitochondrial) LLDDSLVSI X A*02:01 (8 nM)
RAN (GTP-binding nuclear protein Ran) AQYEHDLEVA X A*02:01 (303 nM)
KLIGDPNLEFV X X A*02:01 (5 nM)
RHOC (Rho-related GTP-binding protein RhoC) FSIDSPDSL X C*03:04 (6 nM)
IEVDGKQVEL X X B*40:01 (29 nM)
RPA1 (replication protein A 70 kDa DNA-binding subunit) DTEFPNFKY X A*01:01 (410 nM)
EVFQNANFRSF X A*26:01 (43 nM)
GAIAAIMQK X A*11:01 (32 nM)
KAYGASKTFGK X X A*03:01 (32 nM), A*11:01 (35 nM)
KVIDQQNGL X No binding predicted
SGMGSTVSK X A*11:01 (31 nM)
VSDFGGRSL X X X X X C*03:03 (438 nM), C*03:04 (438 nM), C*05:01 (89 nM)
RPL10A (replication protein A 70 kDa DNA-binding subunit) FLASESLIKQI X X X X A*02:01 (22 nM)
NMVAKVDEV X X X X A*02:01 (51 nM)
RILGPGLNK X A*03:01 (21 nM)
SLIKQIPRI X X X A*02:01 (36 nM)
STIKFQMKK X A*11:01 (5 nM)
TLYEAVREV X X A*02:01 (9 nM)
RPL19 (60S ribosomal protein L19) HMYHSLYLK X A*03:01 (7 nM)
ILMEHIHKL X X X X X A*02:01 (2 nM)
ILMEHIHKLK X A*03:01 37 (nM)
ILMEHIHKLKA X A*02:01 (19 nM)
KLIKDGLIIRK X A*03:01 (49 nM)
RILMEHIHKLK X A*03:01 (90 nM)
RLASSVLRCGK X A*03:01 (23 nM)
WLDPNETNEI X A*02:01 (79 nM)
RPS2 (40S ribosomal protein S2) KEWMPVTKL X B*40:01 (28 nM)
RPSA (40S ribosomal protein SA) NTDSPLRY X A*01:01 (7 nM)
SART1 (U4/U6.U5 tri-snRNP-associated protein 1) GLLETTVQKV X X A*02:01 (9 nM)
KSMNANTITK X X A*03:01 (61 nM), A*11:01 (12 nM)
RVKAPNKSL X No binding predicted
SFMBT1 (Scm-like with four MBT domains protein 1) ESVMINGKY X A*26:01 (41 nM)
SSDNYEHWLY X A*01:01 (5 nM)
SH3GLB2 (endophilin-B2) EVAPPASGTR X No binding predicted
SOX10 (transcription factor SOX-10) ETAGPQGPPHY X X A*26:01 (13 nM)
EVMSNMETF X A*26:01 (14 nM)
FSYMGPSQRPL X X X C*03:03 (3 nM), C*03:04 (3 nM)
GTAAIQAHY X X No binding predicted
ISKPPGVAL X X C*03:04 (228 nM), C*12:03 (152 nM)
YGHSGQASGLY X No binding predicted
YSDHQPSGPYY X A*01:01 (5 nM)
YTDQPSTSQIAY X A*01:01 (4 nM)
KLADQYPHL X X A*02:01 (6 nM)
KTLGKLWRL X X X A*02:01 (74 nM)
STAT1 (signal transducer and activator of transcription 1-alpha/beta) ALLKDQQPGTFLL X A*02:01 (22 nM)
DSFPMEIRQY X X No binding predicted
DSFPMEIRQYL X No binding predicted
EVVHKIIEL X A*26:01 (211 nM)
KLQELNYNL X X X A*02:01 (6 nM)
QQLDSKFLEQV X X A*02:01 (176 nM)
VLWDRTFSL X X X X A*02:01 (3 nM)
VMLDKQKEL X A*02:01 (201 nM)
SUGT1 (suppressor of G2 allele of SKP1 homolog) ETFTEGQKL X No binding predicted
TOP2A (DNA topoisomerase 2-alpha) KLAEAERVGLHK X A*03:01 (59 nM)
KLDETGNSL X X X A*02:01 (68 nM)
KLDETGNSLK X A*03:01 (153 nM)
KLDETGNSLKV X A*02:01 (29 nM)
SLDKDIVAL X A*02:01 (59 nM)
THFPDETEI X B*38:01 (175 nM)
AEINNIIKI X X B*40:01 (58 nM)
EVTFVPGLY X A*26:01 (12 nM)
FLYDDNQRV X X X X A*02:01 (4 nM)
KIFDEILVNA X A*02:01 (26 nM)
LTYNDFINK X A*11:01 (8 nM)
TP53 (cellular tumor antigen p53) GLAPPQHLIRV X A*02:01 (18 nM)
TTK (dual specificity protein kinase TTK) ESFSGSLGHL X No binding predicted
TYMS (thymidylate synthase) NSELSCQ X No binding predicted
NSELSCQLY X A*01:01 (36 nM)
YMIAHITGL X X A*02:01 (3 nM)
TYR (tyrosinase) EEYNSHQSL X X B*40:01 (42 nM)
EVYPEANAPIGHN X No binding predicted
HTISSDYVIPIGTY X A*26:01 (18 nM)
KQLPEEKQPLL X X No binding predicted
LEEYNSHQSL X X B*40:01 (47 nM)
LLMEKEDYHSL X X X X X A*02:01 (6 nM)
MEKEDYHSL X B*40:01 (69 nM)
NIFDLSAPEKDKFFA X A*02:01 (71 nM)
SSDYVIPIGTY X A*01:01 (23 nM)
YVIPIGTYGQM X A*26:01 (37 nM)
WNK2 (serine/threonine protein kinase WNK2) HTRTPPIIHR X No binding predicted