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. Author manuscript; available in PMC: 2017 Jul 13.
Published in final edited form as: Sci Signal. 2017 Feb 28;10(468):eaaf5967. doi: 10.1126/scisignal.aaf5967

Figure 7. DDiT4L activates Akt.

Figure 7

(A) Phosphorylation (p) of Akt was increased in DDiT4L overexpressing mice, which was reversed after transgene suppression. n=6–9 mice per genotype. One-Way ANOVA, Bonferroni post-test, *p<0.05. Phospho-proteins were normalized to total protein.

(B) Phosphorylation of Akt remained increased in DDiT4L/Tta/Beclin mice. n = 3–6 mice per genotype. One Way ANOVA followed by Bonferroni posttest. *p<0.05. Phospho-proteins were normalized to total protein.

(C) Phosphorylation of Akt was increased in NRVMs infected with Ad-DDiT4L (AD-DD), with no change seen with DDiT4L knockdown (DD-KD). n=4–6 preparations, 2 samples per preparation. One Way ANOVA followed by Bonferroni posttest *p<0.05 compared to control. Phospho-proteins were normalized to total protein.

(D) Knockdown of Rictor reduced Ad-DDiT4L infection (Ad-DD) from increasing the phosphorylation of Akt. n = 4 preparations, 2 samples per preparation. One Way ANOVA followed by Bonferroni posttest, *p<0.05, **p<0.01, ***p<0.001 compared to control or indicated sample.

(E) Phosphorylation of Akt was increased in Ad-DDiT4L-infected (Ad-DD) NRVMs, which was blunted after knockdown of TSC2. n=4 preparations, 2 samples per preparation. One Way ANVOA followed by Bonferroni posttest, ***p<0.001, ****p<0.0001 compared to control or indicated sample. Phospho-proteins were normalised to total protein.

(F) Knockdown of Raptor increased phosphorylation of Akt, which was not further increased with Ad-DDiT4L infection (Ad-DD). n=3 preparations, 2 samples per preparation. One Way ANOVA followed by Bonferroni posttest, *p<0.05, ***p<0.001 compared to control or indicated sample. Phospho-proteins were normalised to total protein.