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. Author manuscript; available in PMC: 2018 Jun 1.
Published in final edited form as: J Immunol. 2017 May 1;198(11):4448–4457. doi: 10.4049/jimmunol.1601089

Figure 2. Live GBS and antibiotic-killed GBS induce activation of PKD1 through an MyD88-dependent manner.

Figure 2

Peritoneal macrophages isolated from wild-type (WT) or MyD88−/− mice were stimulated with medium (med), PMA (10 ng/ml), CpG DNA (12 μg/ml), PGN (1 μg/ml), live GBS (108 cfu/ml), or antibiotic-killed GBS (GBS+P/G; 108 cfu of GBS were treated with 1 mg of penicillin G for 6 h) for 45 min. Activation status of PKD1 and JNK was detected by phospho-specific Western blot assay. Actin was used as a loading control. Data represent results obtained from three separate experiments.