Fig 4. Survival of hamsters immunized with purified recombinant Lig proteins after lethal challenge.

Groups of 5–8 female hamsters were immunized subcutaneously three times with 100 μg LigA7’-13 (●), LigB0-7 (■), LigA7’-13 + LigB0-7 (◆), or controls PBS (○) and PBS with adjuvant (□). Animals were challenged intraperitoneally with 1 x 10⁴ L. interrogans at day 0 and post-challenge survival was followed until day 28. Asterisks indicate significant difference between mortality rates compared (Fisher’s exact test, *P < 0.05, ***P < 0.001, ns not significant).