Table 2. List of primers for the differentially expressed genes and the reference genes.
| Gene name | Sequence(5’-3’) | Amplicon size (bp) | PCR efficiency (%) | GenBank accession numbers |
|---|---|---|---|---|
| Target genes | ||||
| sod1 | CCACGTCCATGCCTTTGGR-F | 141 | 95.3 | AY736282.1 |
| TCAGCTGCTGCAGTCACGTT-R | ||||
| il17d | CTTGTCTCCCTGGGCATACAG-F | 201 | 112.7 | EU689087.1 |
| CAATATGCCTCGGGTATGAACCT-R | ||||
| igt | CAACACTGACTGGAACAACAAGGT-F | 97 | 107.7 | GQ907004 |
| CGTCAGCGGTTCTGTTTTGGA-R | ||||
| Reference genes | ||||
| ef1ab | TGCCCCTCCAGGATGTCTAC-F | 59 | 96 | BG933853 |
| CACGGCCCACAGGTACTG-R | ||||
| rpl13 | CGCTCCAAGCTCATCCTCTTCCC-F | 79 | 96.4 | BT048949.1 |
| CCATCTTGAGTTCCTCCTCAGTGC-R | ||||
| rps29 | GGGTCATCAGCAGCTCTATTGG-F | 167 | 94.5 | BT043522.1 |
| AGTCCAGCTTAACAAAGCCGATG-R | ||||
| ubi | AGCTGGCCCAGAAGTACAACTGTG-F CCACAAAAAGCACCAAGCCAAC-R | 162 | 92.7 | AB036060.1 |