Table 8. Differentially expressed distal intestinal proteins of Atlantic salmon fed microalga feeds for 84 days.
| Spot no. | Protein name | Apparent pI/MW (kDa) | Peptide sequenceda |
|---|---|---|---|
| 1 | Alpha-2-HS-glycoprotein-like, Ahsg | 3.0/88.5 | YALNQIDDIK VVTAVEGDCDVVLR ESLFAIMEVGR |
| 2↑ | Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial-like, P5cdh | 7.9/88.6 | NEPILGFNEGSPER AADIISGPK TVVQAEIDAAAELIDFFR HAVELESQQPLDSDGSTNTMLYR QVAQNLDVYK SADVQSVVTGTIR STGSIVAQQPFGGAR |
| 3↑ | Dihydrolipoyl dehydrogenase, mitochondrial-like, Dld | 8.16/87.8 | NQVTATAEDGSMQVINSK RPDGQIDVAVEAAAGGK NLGLDTVGLELDNR VPSIYAIGDVIAGPMLAHK FPFAANSR |
| 4 | Leukocyte elastase inhibitor-like, Lei | 6.0/68.8 | TGNVFYSPLSISSALAMVSLGAR ATDNVHVGFNK GAPYALSLANR LYGEQSYQFVETFLGDTK KHYNAELEAVDFK HYNAELEAVDFK NLLAEGVVDHLTR LVLVNAIYFK FKESSTSDALFK ESSTSDALFK NLVEWTRPDMMDTVEVQVGLPK FKLEESLDLK SDFSGMSPNNDLVLSK AFVEVNEEGTEAAGATAAIMMMR |
| 5 | Creatine kinase B-type isoform X2, Crt | 6.7/60.7 | ILTPAIYER ELLDPIIEDR MSVEALDSLSGDLK GTGGVDTAAVGGTFDISNADR LGFSEVELVQMVVDGVK GQSIDDLMPAQK |
| 6 | Elongation factor 2, Ef2 | 6.6/40.3 | AKPFPDGLAEDIEK EGVLCEENMR TAIVVAETR |
| 7 | Apolipoprotein A-I-1 precursor, Apoa1-1 | 5.6/29.1 | AALNMYIAQVK SIDLLDDTEYK SIDLLDDTEYKEYK SLAPYTTVFGTQLADATATVR AKIEPVVEEMR IEPVVEEMR VAVNVEETK LMPIVEIVR LMPIVEIVR TLAAPYAEEYKEQMFK |
| 8↑ | Triosephosphate isomerase, Tpi | 7.6/28.8 | IGVAAQNCYK GGAFTGEISPAMIK VVLAYEPVWAIGTGK ANVSEAVANSVR DVDGFLVGGAALKPEFVDIINAK |
| 9↑ | Flavin reductase, Flr | 8.1/29.2 | TMQGQDAVIIILGTR LLPVTEDHDR ESGLDFVAVMPPHIDDNFPLTEK |
aUnique peptides are in bold; indicates underexpression and ↑ indicates overexpression in the algal groups compared to the control group