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. 2017 Jul 13;12(7):e0180988. doi: 10.1371/journal.pone.0180988

Fig 3. Ligo-miR EZ specificity data.

Fig 3

a) Single-plex synthetic miRNAs were analyzed using Ligo-miR EZ Panel B. No cross-talk is seen across unrelated miRNA. Cross-talk is only seen in Lanes 11 and 18 which represent miR-17-5p and miR-106a-5p that differ by only a single nucleotide at the 5’ end. b) An 8-plex let-7 family probe set was used to test assay specificity. Lanes 1 (left) and 10 (right) contain all 8-plex miRNA while Lanes 2–9 (l—r) have 1-plex miRNAs. Limited cross-talk is seen across probe-miRNA pairs with sequence mismatches distant to the ligation site. c) Let-7a precursor and mature miRNA were input into a single-plex let-7a assay. No response was seen even with a high background of precursor molecules. ++ = 1500 attomoles, + = 150 attomoles,— = no miRNA.