Fig 1. L. major transitions from neutrophils to inflammatory monocytes during acute infection.

C57BL/6 CX₃CR1-gfp mice were injected with 2x10⁵ L. major-RFP parasites i.d. in the ear. (A-D) Representative plots of blood or skin cells stained with the indicated markers. (A-C) CX₃CR1 and Ly6C expression on total (A and B) or RFP⁺ (C) CD11b⁺ cells including Ly6G expression on the CX₃CR1^-Ly6C^int population (lower panels). Mono.: Inflammatory Monocytes; Neut.: Neutrophils. (D) Representative plots of Live cells (gates as per S1A Fig) from the skin on day 3 p.i.. (E-J) Kinetic analysis of total (E-G) or RFP⁺ (H-J) CD11b⁺ populations in the skin. (E, G, H, and I) Enumeration of cells using counting beads. (F and I) Frequency of the indicated subsets within total CD11b⁺ (F) or RFP⁺CD11b⁺ (I) cells. (G and J) Total (G) or RFP⁺ (J) number of neutrophils (Neut.) or Ly6C⁺CCR2⁺CX3CR1^+/- inflammatory monocytes (Infl. Mono.) per ear. Data are from one experiment representative of more than 3 similar experiments. Data are the mean. n = 4 (0h), 6 (2h, 10h, 2d) or 10 (7d) ears per group. In E, F, H, and I the asterisks indicate a significant difference between the time points referred to by the brackets. In G and J the symbols indicate a significant difference between the numbers of neutrophils (*) or monocytes (#) at the time points referred to by the brackets. The level of significance is indicated in the materials and methods.