Fig 5. Immature inflammatory monocytes are the predominant iNOS⁺ cell type at sites of L. major challenge.

Mice as described in Fig 4 were analyzed for iNOS expression on day 3 post-ch. with L. major-RFP. (A) Representative histogram and plots of iNOS⁺ or iNOS^- cells. (B) % or (C) absolute number of CD11b⁺ cells that are iNOS⁺ on day 3 post-ch. (D) Frequency of the indicated populations within the total iNOS⁺CD11b⁺ population. (E) Percentage of the indicated populations on the x-axis that are iNOS⁺. (F) MHCII, CD11c, CD86 MFI of, and % of CD11c⁺MHCII^hi cells within iNOS⁺ or iNOS^- monocytes obtained from the same site of infection. (G) Representative RFP and NOS2 staining of the population described in (F). (H) Percentage of iNOS⁺ or iNOS^- monocytes gated as in (F) that are RFP⁺. (I) Percentage of RFP⁺ or RFP^- monocytes gated as in (F) that are iNOS⁺. (J) iNOS MFI of iNOS-producing RFP^- versus RFP⁺ monocytes gated as in (F). Each paired data set represents cells from the same ear. (K) Percentage of the indicated populations within the total CD11b⁺ infected iNOS⁺ versus infected iNOS^- populations. Data are from one experiment representing 3 similar experiments.