Fig 6. Interferon-γ drives chemokine expression, monocyte recruitment, iNOS-production and parasite killing at sites of secondary challenge.

Mice as described in Fig 4 were treated with 0.5mg i.p. anti-IFN-γ or control antibody on day -1 and analyzed on day 4 post-ch. (A) Parasite load per ear determined by LDA. (B and C) Frequency (B) or absolute number (C) of CD11b⁺ cells per ear. (D) Frequency of the indicated populations within the total CD11b⁺ population per ear. (E) Absolute number of the indicated monocyte populations per ear. (F and G) Frequency of iNOS⁺ within total CD11b⁺ cells (F) and absolute number of CD11b⁺iNOS⁺ cells (G) per ear. (H) Absolute number of the indicated iNOS⁺ monocyte populations per ear. (I) Frequency of iNOS⁺ cells within the total indicated monocyte populations. (J) Gene expression was quantified as reported in Fig 3J. (K) MHCII MFI of RFP⁺ or RFP^- monocytes. (L) Frequency of CD11c⁺MHCII^hi cells within the indicated populations. Data are from one experiment representing 4 similar experiments. Data in (J) are pooled from two similar experiments in which qPCR was performed, n = 6 mice per condition.