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. 2017 Jul 11;474(14):2449–2464. doi: 10.1042/BCJ20160825

Figure 5. HIV-1 Tat induces nuclear-cytoplasmic re-distribution of Mdm2.

Figure 5.

(a) and (b) MCF-7 cells were transfected with 1 µg HA-Tat. Nuclear and cytoplasmic fractions were prepared 36 h post-transfection using NE-PER® Nuclear and Cytoplasmic Extraction Reagents Kit (Thermo Scientific). After preparing the fractions, western blot analysis was performed. LaminB2 was used as a loading control in the nuclear fraction whereas GAPDH was used as a loading control in the cytosolic fraction. (c) Relative protein expression in each fraction was calculated using Image J software. The results are representative of three independent experiments. P-values were calculated by a two-tailed t-test (*P < 0.05, **P < 0.01).