FIGURE 2:

Intact Sse1 SBD function is not obligate for the Hsp70/Hsp40/Hsp110 machine to disaggregate or refold substrates. (A) FFL refolding over time in the presence of different chaperone mixtures as indicated in the schematic and described in detail in Materials and Methods. (B) FFL disaggregation and refolding over time in the presence of the same chaperone mixtures shown in A, as indicated in the schematic and described in detail in Materials and Methods. Human proteins HSPA8, DnaJB1, and HSPH2 are labeled as Hsp70, Hsp40, and Hsp110, respectively, in the experimental schematic and as 70, 40, and 110 in the plots. Yield is calculated as the percentage reactivation of luciferase relative to activity before denaturation.