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. Author manuscript; available in PMC: 2018 May 22.
Published in final edited form as: Anal Chim Acta. 2017 Mar 16;968:40–49. doi: 10.1016/j.aca.2017.03.003

Figure 3.

Figure 3

Number of PSMs identified from duplicates of DiLeu-labeled HEK293 cell lysate tryptic peptides according to HCD NCE (A) and EThcD NCE (B). Although most IDs using regular HCD are found at an NCE of 30, EThcD requires elevated NCE to generate sufficient fragmentation for identification.