Figure 1.
A BarSeq Screen for Parasite Growth Rate
(A) Schematic illustration of screen design.
(B) Circos plot showing, from the outer to inner circle, chromosome, mutant RGR (color coded by phenotype as in E), extent of previous phenotyping as reported on RMgmDB, and coverage in our screen.
(C) Screenshot of the PlasmoGEM phenotype viewer showing RGR measurements plotted against confidence expressed as the negative logarithm of their variance. See (E) for color coding of phenotypes.
(D) Frequency distribution of the >50 RGR replicates available for six control genes compared to essential ribosomal genes.
(E) Asexual growth phenotypes defined using confidence intervals (CI) and illustrated with representative genes.
(F) Graph on the left showing percentage of genes with at least one RGR measurement at a given confidence limit (black circles) and how confidence relates to experimental reproducibility (blue squares). Reproducibility of independent duplicates is illustrated by the regression plot on the right for confidence 4 or above, a level reached by at least one measurement for 85% of genes in the screen.