Skip to main content
. Author manuscript; available in PMC: 2017 Jul 14.
Published in final edited form as: Nat Struct Mol Biol. 2014 Jun 15;21(7):585–590. doi: 10.1038/nsmb.2842

Figure 2.

Figure 2

Canonical microRNA pathway and miR-295 are required for lncRNA expression. (a) Schematic of the canonical miRNA pathway. (b) NanoString measurement of expression of 102 lncRNAs in three lines of Dcr-KO mESCs (normalized to WT), Dcr rescue in Dcr KO (normalized to the catalytically dead mutants), Dgcr8 KO (normalized to Dgcr8 WT) and miR-295 rescue in Dcr KO (normalized to a control miRNA mimic). The box on the left shows the ability of lncRNAs to maintain pluripotency (column 1) and to prevent differentiation (column 2) as well as their binding to chromatin (column 3), as cross-referenced with data from ref. 10. The blue and yellow colors in the heat map indicate down- and upregulated lncRNAs respectively (n = 2 cultures each).