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. 2017 Jul 13;7:5315. doi: 10.1038/s41598-017-04735-0

Figure 3.

Figure 3

rhPAPPA up-regulates the expression of FUT1, FUT4 and FUT8 in HEC-1A cells. HEC-1A cells were treated with rhPAPPA (1 ng/ml and 10 ng/ml) for 48 h or rhPAPPA (10 ng/ml) for 24 h, 48 h and 72 h, respectively. (A,B) q-PCR detected the mRNA levels of FUT1, FUT4 and FUT8. (C,D) Immunofluorescent staining detected the cellular localization and (E,F) Western blotting analyzed the protein levels of FUT1, FUT4 and FUT8. GAPDH was used as an internal control. The bar represents 50 μm. *p < 0.05, **p < 0.01, ***p < 0.001. The data were presented as the means ± SEM of three independent experiments.