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. 2017 Jul 13;7:5315. doi: 10.1038/s41598-017-04735-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rhPAPPA stimulates the expression of FUT1/4/8 through the IGF-1R/PI3K/Akt signaling pathway. Ishikawa cells were differently treated with rhPAPPA, AG1024, LY294002. (A) Westerrn blotting showed the expression of IGF-1R, p-IGF-1R (Tyr¹¹³¹), Akt, p-Akt (Tyr³⁰⁸) and p-Akt (Ser⁴⁷³). (B,C) Western blotting analyzed the molecules of the IGF-1R/PI3K/Akt signaling pathway and FUT1/4/8 expression. (D) Immunofluorescent staining of FUT1/4/8. DAPI (blue) was used for nuclear staining. GAPDH was used as an internal control. The bar represents 50 μm. The data were presented as the means ± SEM of three independent experiments.