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. 2017 Jul 13;7:5315. doi: 10.1038/s41598-017-04735-0

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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rhPAPPA promotes endometrium receptivity by up-regulating the specific fucosylation of integrin αVβ3. (A) Adhesion of JAR cells to Ishikawa cells pre-treated without (a) untreated control, or with (b) IgG, (c) anti-αV, (d) anti-β3, (e) anti-αVβ3, (f) rhPAPPA + IgG, (g) rhPAPPA + anti-αVβ3, (h) rhPAPPA + anti-αVβ3 + UEA-1, (i) rhPAPPA + anti-αVβ3 + LTL, (j) rhPAPPA + anti-αVβ3 + LCA. (B) Adhesion rate of JAR cells to pre-treated Ishikawa as indicated is shown in the histogram. (C) Integrin αVβ3 was immunoprecipitated from the whole-protein lysate of untreated control and rhPAPPA-treated Ishikawa cells. The specific N-fucosylation of αVβ3 was detected by Western blotting. αV and β3 were detected to show the loading protein amount. Input showed the efficiency of immunoprecipitation. (D) Immunofluorescence and Lectin staining detected the expression and cellular localization of the specific fucosylation and αVβ3 after rhPAPPA treatment. Green, α1,2-, α1,3-, or α1,6-fucosylation; red, αVβ3; yellow (overlay), co-staining of α1,2-, α1,3-, or α1,6-fucosylation with αVβ3. DAPI (blue) was used for nuclear staining. The bar represents 50 μm. **p < 0.01, ***p < 0.001. The data were presented as the means ± SEM of three independent experiments.