Figure 6.
Transcript levels of genes involved in cellulose (Upp, CesA8, and CesA122-1) (a) and fatty acid (Fas1a and Pksa), lipid (Dgat-2), and cellulose (CesA8) (b) biosynthesis in N. salina cells grown under N+ and N− conditions for 2 days. In (b), transcription inhibitor actinomycin D (AD, 100 μg ml−1) was included in N− media for 2 days prior to the onset of N− growth. Absolute copy numbers of the mRNA transcripts (number of copies/ng of total RNA) were determined by qRT-PCR analysis. Each data point represents the mean ± SE of three biological and technical triplicate culture flasks.