Table 2.

Changes in neutral lipid and total fatty acid (TFA) contents, and fatty acid compositions from N. salina cells grown under nitrogen-rich (N+) and nitrogen-depleted (N−) conditions for 2 days.

Sample	Neutral lipid (F580)	TFA (g/g DW)	Fatty acid composition (%)
			Palmitic acid (C16:0)	Palmitoleic acid (C16:1)	Oleic acid (C18:1)	ETA (C20:4ω6)	EPA (C20:5ω3)
N+	9.98 ± 0.91	0.17 ± 0.02	21.18 ± 0.11	33.04 ± 0.61	2.01 ± 0.07	7.70 ± 0.36	16.27 ± 0.58
N−	67.09 ± 2.95	0.34 ± 0.03	35.43 ± 0.21	39.45 ± 0.14	5.47 ± 0.03	2.16 ± 0.14	4.87 ± 0.18

Neutral lipid content was estimated by Nile Red fluorescence amplitude at 580 nm excited at 430 nm. Only significantly different fatty acid species between the two samples are represented. Data are expressed as the average of three biological replicates ± SD. Each sample was significantly different from the other (independent two-tailed t-test, p-value < 0.05). EPA, eicosapentaenoic acid; ETA, eicosatetraenoic acid; TFA, total fatty acid.