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. 2017 Jul 13;7:5324. doi: 10.1038/s41598-017-05641-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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EPO purity after affinity purification. From left to right: Eluate of the affinity purification from CCS (triplicate); Eluate without prior application of CCS (negative control); EPO standard in ascending order of amounts (1, 2 and 5 µg). The three different bands of each affinity purification of CCS were excised and subjected to a standard protein identification workflow (see Supporting Information Table S3).