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. 2017 Jul 13;7:5268. doi: 10.1038/s41598-017-05652-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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qRT-PCR-based expression analyses directly comparing the atrial differentiation protocol that employed RA (atrial-CM, grey bars) and ventricular differentiation protocol (ventricular-CM, black bars). Cell populations were analyzed at T20 of the differentiation protocol. The RA protocol was able to generate cells that are enriched in atrial specific markers (NPPA, KCNJ3, and GJA5) and lacked ventricular markers (MYL, IRX4). Values are shown relative the housekeeping gene TBP. Error bars represent standard deviation of the mean from the values of independent experiments (N ≥ 4); *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.