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. 2017 Jul 13;7:5268. doi: 10.1038/s41598-017-05652-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescent immunostaining for the presence of troponin and vimentin in cell sheets generated from the atrial differentiation protocol. DAPI staining shows cell nuclei. Troponin staining demonstrates disorganized ultrastructural arrangement of cardiomyocytes. Vimentin staining showing infrequent and isolated mesenchymal cells. Scale bar is 50 μm.