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. 2017 Jul 13;7:5280. doi: 10.1038/s41598-017-05709-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Levels of ceramides are increased in cortical tissue from R6/2 mice at early stages of the disease. Representative dot blotting and densitometric analysis (A) and immunohistochemical micrograph. Scale bar in each micrograph represents 100 µm. (B) of Cer content in the cortex of early manifest (6 week old) R6/2 mice and WT littermates. Data are represented as mean ± SD. N = 5 for each group of mice. *p < 0.05 (Unpaired t-test).