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. 2017 Jul 13;7:5279. doi: 10.1038/s41598-017-05433-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Primary human RPE cells. (a) Confocal images of immunostained primary human RPE cells using specific markers ZO-1 (Zona occludens-1), RPE65 (retinal pigment epithelium-specific 65 kDa protein), MITF (Microphtalmia-associated Transcription Factor), and β-catenin. (b) Light microscopy depicting primary human RPE cell morphology at different passages in culture. Scale bars, 50 μm.