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. 2017 Jul 13;7:5279. doi: 10.1038/s41598-017-05433-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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32:6n3 and 34:6n3 ameliorate UOS-induced primary human RPE cell death. (a) Untreated (control) RPE cells. RPE cells underwent UOS for 16 hours (b-f). When 32:6n3 or 34:6n3 were added, cell death was prevented (c,d,f). Typical fields of cell cultures are represented in the right column. Nuclei are labeled with Hoechst staining, and the dead cells are highlighted in green. These were separated using an intensity threshold algorithm and counted using an Image J macro (left column)⁷⁰. (e) Quantification of live (control cells; black curve) and dead (UOS cells, red curve) cells was based on nuclear size⁷⁰. Error bars, SEM; *p < 0.05.