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. 2017 Jul 13;7:5282. doi: 10.1038/s41598-017-05682-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of the VMH nucleus is normal in the adult COUP-TFII mutant. (A–D) The expression of COUP-TFII (green) is readily detected in the majority of SF1⁺ VMH neurons (red) at 1 M. (E–L) The expression of COUP-TFII (green) in the VMH nucleus was not altered between the control and the mutant at 3 M; additionally, there were few LacZ signals (red) in the VMH nucleus of the control and the mutant. VMH, ventromedial nucleus of hypothalamus. Scale bars, (A–D,E–L) 200 μm.