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. 2017 Jul 13;7:5270. doi: 10.1038/s41598-017-05560-1

Figure 2.

Figure 2

Effects of miR-30a mimics on the mRNA and protein expressions of inflammatory factors. (a–f) RT-PCR showing miR-30a mimics significantly inhibited the increased mRNA expressions of Il-1a, Il-6 and TNF-a in LPS-induced RAW264.7 and LRM-induced Beta-TC-6 cells. (g–o) ELISA showing that miR-30a mimics significantly inhibit the increased protein levels of IL-1α, IL-6 and TNF-α in RAW264.7 cells after 24 h of LPS induction (samples from cell medium and lysis) as well as in Beta-TC-6 cells after 24 h of LRM induction (samples from cell lysis only). LPS+/−, incubation with or without LPS; LRM+/−, incubation with or without LRM; NC, negative controls of miRNA mimics; NC-i, negative controls of miRNA inhibitors or siRNA; miR-30a-i, miRNA-30a inhibitor; and si-IL1a, silencing mRNA fragment of Il-1a. Data are expressed as the Mean ± SD (n = 3); *P < 0.05 and **P < 0.01 vs. NC or NC-i (LPS/LRM+), respectively.