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. 2017 Jul 13;7:5270. doi: 10.1038/s41598-017-05560-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of miR-30a mimics on apoptosis in RAW264.7 and Beta-TC-6 cells subjected to inflammatory stimulation measured by flow cytometry. (a and c) Flow cytometry showing that miR-30a mimics significantly inhibit the increased apoptosis in LPS-induced RAW264.7 cells. (b and d) Flow cytometry showing that miR-30a mimics significantly inhibited the increased apoptosis in LRM-induced Beta-TC-6 cells. LPS+/−, incubation with or without LPS; LRM+/−, incubation with or without LRM; NC, negative controls of miRNA mimics; NC-i, negative controls of miRNA inhibitors or siRNA; miR-30a-i, miRNA-30a inhibitor; and si-IL1a, silencing mRNA fragment of Il-1a. Data are expressed as the Mean ± SD (n = 3); **P < 0.01 vs. NC or NC-i (LPS/LRM+), respectively.