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. 2017 Jul 13;7:5260. doi: 10.1038/s41598-017-05413-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunopatterns of H4K16ac (a1–a4) and H3K9ac (b1–b4) in the nuclei isolated from B. hybridum roots. a1,b1–DAPI counterstained nuclei; a2,b2–histone immunopatterns; a3,b3–superimposed channels of particular histone modification foci distribution (red) and DAPI (blue); a4,b4–schematic representation of H4K16ac (a4) and H3K9ac (b4) foci distribution in the nucleolus and adjacent chromocentres. Nu – nucleolus. Scale bar: 5 µm.