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. 2017 Jul 13;7:5260. doi: 10.1038/s41598-017-05413-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of heterochromatic marker H3K9me2 in the nuclei of B. hybridum. (a) H3K9me2 immunopattern in the chromocentres adjacent to the nucleolus. (b1–b3; c1–c3) H3K9me2 foci distribution in the nucleus without distinct heterochromatic domains. Three example sections through the representative nucleus are presented. (b1–b3) FISH with 25S rDNA (red) as a probe. (c1–c3) H3K9me2 foci (green) distribution and 25S rDNA FISH signals (red) in the sections through nucleus presented on (b1–b3). (d1–d3) H3K9me2 foci distribution in the nucleus with chromocentres. (d1) FISH with 25S rDNA (red) as a probe. (d2) H3K9me2 foci distribution in the nucleus shown on (d1). (d3) H3K9me2 foci (green) distribution and 25S rDNA hybridisation signals (red) in the nucleus presented on (d1). The locations of B. stacei-originated 35S rDNA loci are indicated by yellow insets. Nu–nucleolus. Scale bar: 5 µm.