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. 2017 Jul 13;7:5303. doi: 10.1038/s41598-017-05525-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Conjugation of folic acid to His-tagged VLNPs. (a) Spectra of folic acid (FA), HisHBcAg nanoparticles (HisHBcAg), and FA-conjugated HisHBcAg nanoparticles (FA-HisHBcAg). (b) Electron micrographs of HisHBcAg nanoparticles. The samples (as labelled on top of the micrographs) were stained with uranyl acetate and viewed under a TEM. All the samples assembled into spherical structures. White bars indicate 50 nm.