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. 1980 May;28(2):577–584. doi: 10.1128/iai.28.2.577-584.1980

Fibril-mediated adherence of Actinomyces viscosus to saliva-treated hydroxyapatite.

T T Wheeler, W B Clark
PMCID: PMC550973  PMID: 7399676

Abstract

Fibril-mediated adherence of Actinomyces viscosus strain T14V cells to saliva-treated hydroxyapatite was studied. Fibrils were purified by ammonium sulfate precipitation and differential centrifugation from the crude supernatant of whole cells that were sheared by one passage through a French pressure cell. Purified fibrils and crude supernatant inhibited strain T14V adherence to saliva-treated hydroxyapatite to similar extents. However, anti-strain T14V serum and antifibril specific antibody completely abolished strain T14V adherence. The blocking immunoglobulin could be adsorbed from anti-T14V serum by strain T14V whole cells, by purified fibrils, and, to a lesser extent, by cell walls. It was concluded that fibrils mediate adherence of strain T14V cells to saliva-treated hydroxyapatite. In addition, fibril preparations were shown to contain more than 95% protein and to be antigenically homogeneous by immunodiffusion and Laurell rocket immunoelectrophoresis. Purified fibril preparations showed serological identity with the virulence-associated 1 antigen of Lancefield-extracted T14V cells, whereas crude supernatants contained both virulence-associated 1 and virulence-associated 2 antigens, as shown by rocket immunoelectrophoresis.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Axén R., Ernback S. Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers. Eur J Biochem. 1971 Feb 1;18(3):351–360. doi: 10.1111/j.1432-1033.1971.tb01250.x. [DOI] [PubMed] [Google Scholar]
  2. BLEIWEIS A. S., KARAKAWA W. W., KRAUSE R. M. IMPROVED TECHNIQUE FOR THE PREPARATION OF STREPTOCOCCAL CELL WALLS. J Bacteriol. 1964 Oct;88:1198–1200. doi: 10.1128/jb.88.4.1198-1200.1964. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brecher S. M., van Houte J., Hammond B. F. Role of colonization in the virulence of Actinomyces viscosus strains T14-Vi and T14-Av. Infect Immun. 1978 Nov;22(2):603–614. doi: 10.1128/iai.22.2.603-614.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brinton C. C., Jr The structure, function, synthesis and genetic control of bacterial pili and a molecular model for DNA and RNA transport in gram negative bacteria. Trans N Y Acad Sci. 1965 Jun;27(8):1003–1054. doi: 10.1111/j.2164-0947.1965.tb02342.x. [DOI] [PubMed] [Google Scholar]
  5. Buchanan T. M. Antigenic heterogeneity of gonococcal pili. J Exp Med. 1975 Jun 1;141(6):1470–1475. doi: 10.1084/jem.141.6.1470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Buchanan T. M., Pearce W. A. Pili as a mediator of the attachment of gonococci to human erythrocytes. Infect Immun. 1976 May;13(5):1483–1489. doi: 10.1128/iai.13.5.1483-1489.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cisar J. O., Kolenbrander P. E., McIntire F. C. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii. Infect Immun. 1979 Jun;24(3):742–752. doi: 10.1128/iai.24.3.742-752.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cisar J. O., Vatter A. E., McIntire F. C. Identification of the virulence-associated antigen on the surface fibrils of Actinomyces viscosus T14. Infect Immun. 1978 Jan;19(1):312–319. doi: 10.1128/iai.19.1.312-319.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cisar J. O., Vatter A. E. Surface fibrils (fimbriae) of Actinomyces viscosus T14V. Infect Immun. 1979 May;24(2):523–531. doi: 10.1128/iai.24.2.523-531.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Clark W. B., Bammann L. L., Gibbons R. J. Ability of Streptococcus mutans and a glucosyltransferase-defective mutant to colonize rodents and attach to hydroxyapatite surfaces. Infect Immun. 1978 Aug;21(2):681–684. doi: 10.1128/iai.21.2.681-684.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Clark W. B., Bammann L. L., Gibbons R. J. Comparative estimates of bacterial affinities and adsorption sites on hydroxyapatite surfaces. Infect Immun. 1978 Mar;19(3):846–853. doi: 10.1128/iai.19.3.846-853.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cuatrecasas P. Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads. J Biol Chem. 1970 Jun;245(12):3059–3065. [PubMed] [Google Scholar]
  13. DUGUID J. P., SMITH I. W., DEMPSTER G., EDMUNDS P. N. Non-flagellar filamentous appendages (fimbriae) and haemagglutinating activity in Bacterium coli. J Pathol Bacteriol. 1955 Oct;70(2):335–348. doi: 10.1002/path.1700700210. [DOI] [PubMed] [Google Scholar]
  14. Dazzo F. B., Brill W. J. Bacterial polysaccharide which binds Rhizobium trifolii to clover root hairs. J Bacteriol. 1979 Mar;137(3):1362–1373. doi: 10.1128/jb.137.3.1362-1373.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Ellen R. P., Balcerzak-Raczkowski I. B. Interbacterial aggregation of Actinomyces naeslundii and dental plaque streptococci. J Periodontal Res. 1977 Jan;12(1):11–20. doi: 10.1111/j.1600-0765.1977.tb00104.x. [DOI] [PubMed] [Google Scholar]
  16. Ellen R. P., Gibbons R. J. M protein-associated adherence of Streptococcus pyogenes to epithelial surfaces: prerequisite for virulence. Infect Immun. 1972 May;5(5):826–830. doi: 10.1128/iai.5.5.826-830.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ellen R. P., Walker D. L., Chan K. H. Association of long surface appendages with adherence-related functions of the gram-positive species Actinomyces naeslundii. J Bacteriol. 1978 Jun;134(3):1171–1175. doi: 10.1128/jb.134.3.1171-1175.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Fader R. C., Avots-Avotins A. E., Davis C. P. Evidence for pili-mediated adherence of Klebsiella pneumoniae to rat bladder epithelial cells in vitro. Infect Immun. 1979 Aug;25(2):729–737. doi: 10.1128/iai.25.2.729-737.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Gibbons R. J., Houte J. V. Bacterial adherence in oral microbial ecology. Annu Rev Microbiol. 1975;29:19–44. doi: 10.1146/annurev.mi.29.100175.000315. [DOI] [PubMed] [Google Scholar]
  20. Girard A. E., Jacius B. H. Ultrastructure of Actinomyces viscosus and Actinomyces naeslundii. Arch Oral Biol. 1974 Jan;19(1):71–79. doi: 10.1016/0003-9969(74)90228-3. [DOI] [PubMed] [Google Scholar]
  21. Hammond B. F., Steel C. F., Peindl K. S. Antigens and surface components associated with virulence of Actinomyces viscosus. J Dent Res. 1976 Jan;55:A19–A25. doi: 10.1177/002203457605500111011. [DOI] [PubMed] [Google Scholar]
  22. Hay D. I., Gibbons R. J., Spinell D. M. Characteristics of some high molecular weight constituents with bacterial aggregating activity from whole saliva and dental plaque. Caries Res. 1971;5(2):111–123. doi: 10.1159/000259739. [DOI] [PubMed] [Google Scholar]
  23. Honda E., Yanagawa R. Agglutination of trypsinized sheep erythrocytes by the pili of Corynebacterium renale. Infect Immun. 1974 Dec;10(6):1426–1432. doi: 10.1128/iai.10.6.1426-1432.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Isaacson R. E., Fusco P. C., Brinton C. C., Moon H. W. In vitro adhesion of Escherichia coli to porcine small intestinal epithelial cells: pili as adhesive factors. Infect Immun. 1978 Aug;21(2):392–397. doi: 10.1128/iai.21.2.392-397.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. JORDAN H. V., KEYES P. H. AEROBIC, GRAM-POSITIVE, FILAMENTOUS BACTERIA AS ETIOLOGIC AGENTS OF EXPERIMENTAL PERIODONTAL DISEASE IN HAMSTERS. Arch Oral Biol. 1964 Jul-Aug;9:401–414. doi: 10.1016/0003-9969(64)90025-1. [DOI] [PubMed] [Google Scholar]
  26. James-Holmquest A. N., Swanson J., Buchanan T. M., Wende R. D., Williams R. P. Differential attachment by piliated and nonpiliated Neisseria gonorrhoeae to human sperm. Infect Immun. 1974 May;9(5):897–902. doi: 10.1128/iai.9.5.897-902.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Jordan H. V., Fitzgerald R. J., Stanley H. R. Plaque formation and periodontal pathology in gnotobiotic rats infected with an oral actinomycete. Am J Pathol. 1965 Dec;47(6):1157–1167. [PMC free article] [PubMed] [Google Scholar]
  28. Jordan H. V., Keyes P. H., Bellack S. Periodontal lesions in hamsters and gnotobiotic rats infected with actinomyces of human origin. J Periodontal Res. 1972;7(1):21–28. doi: 10.1111/j.1600-0765.1972.tb00627.x. [DOI] [PubMed] [Google Scholar]
  29. Kröll J. Immunochemical identification of specific precipitin lines in quantitative immunoelectrophoresis patterns. Scand J Clin Lab Invest. 1969 Aug;24(1):55–60. doi: 10.3109/00365516909080132. [DOI] [PubMed] [Google Scholar]
  30. Kumazawa N., Yanagawa R. Chemical properties of the pili of Corynebacterium renale. Infect Immun. 1972 Jan;5(1):27–30. doi: 10.1128/iai.5.1.27-30.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Kumazawa N., Yanagawa R. Comparison of the chemical and immunological properties of the pili of three types of Corynebacterium renale. Jpn J Microbiol. 1973 Jan;17(1):13–19. doi: 10.1111/j.1348-0421.1973.tb00698.x. [DOI] [PubMed] [Google Scholar]
  32. LANCEFIELD R. C., PERLMANN G. E. Preparation and properties of type-specific M antigen isolated from a group A, type 1 hemolytic streptococcus. J Exp Med. 1952 Jul;96(1):71–82. doi: 10.1084/jem.96.1.71. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  34. Laurell C. B. Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem. 1966 Apr;15(1):45–52. doi: 10.1016/0003-2697(66)90246-6. [DOI] [PubMed] [Google Scholar]
  35. Loesche W. J., Syed S. A. Bacteriology of human experimental gingivitis: effect of plaque and gingivitis score. Infect Immun. 1978 Sep;21(3):830–839. doi: 10.1128/iai.21.3.830-839.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. McIntire F. C., Vatter A. E., Baros J., Arnold J. Mechanism of coaggregation between Actinomyces viscosus T14V and Streptococcus sanguis 34. Infect Immun. 1978 Sep;21(3):978–988. doi: 10.1128/iai.21.3.978-988.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Nagy B., Moon H. W., Isaacson R. E. Colonization of porcine intestine by enterotoxigenic Escherichia coli: selection of piliated forms in vivo, adhesion of piliated forms to epithelial cells in vitro, and incidence of a pilus antigen among porcine enteropathogenic E. coli. Infect Immun. 1977 Apr;16(1):344–352. doi: 10.1128/iai.16.1.344-352.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. OSSERMAN E. F. A modified technique of immunoelectrophoresis facilitating the identification of specific precipitin arcs. J Immunol. 1960 Jan;84:93–97. [PubMed] [Google Scholar]
  39. Ottow J. C. Ecology, physiology, and genetics of fimbriae and pili. Annu Rev Microbiol. 1975;29:79–108. doi: 10.1146/annurev.mi.29.100175.000455. [DOI] [PubMed] [Google Scholar]
  40. Powell J. T., Fischlschweiger W., Birdsell D. C. Modification of surface composition of Actinomyces viscosus T14V and T14AV. Infect Immun. 1978 Dec;22(3):934–944. doi: 10.1128/iai.22.3.934-944.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Salit I. E., Gotschlich E. C. Hemagglutination by purified type I Escherichia coli pili. J Exp Med. 1977 Nov 1;146(5):1169–1181. doi: 10.1084/jem.146.5.1169. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Salit I. E., Gotschlich E. C. Type I Escherichia coli pili: characterization of binding to monkey kidney cells. J Exp Med. 1977 Nov 1;146(5):1182–1194. doi: 10.1084/jem.146.5.1182. [DOI] [PMC free article] [PubMed] [Google Scholar]
  43. Smith D. J., Taubman M. A., Ebersole J. L. Effect of oral administration of glucosyltransferase antigens on experimental dental caries. Infect Immun. 1979 Oct;26(1):82–89. doi: 10.1128/iai.26.1.82-89.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  44. Swanson J. Studies on gonococcus infection. IV. Pili: their role in attachment of gonococci to tissue culture cells. J Exp Med. 1973 Mar 1;137(3):571–589. doi: 10.1084/jem.137.3.571. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Syed S. A., Loesche W. J. Bacteriology of human experimental gingivitis: effect of plaque age. Infect Immun. 1978 Sep;21(3):821–829. doi: 10.1128/iai.21.3.821-829.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  46. Taubman M. A., Smith D. J. Effects of local immunization with glucosyltransferase fractions from Streptococcus mutans on dental caries in rats and hamsters. J Immunol. 1977 Feb;118(2):710–720. [PubMed] [Google Scholar]
  47. Wheeler T. T., Clark W. B., Birdsell D. C. Adherence of Actinomyces viscosus T14V and T14AV to hydroxyapatite surfaces in vitro and human teeth in vivo. Infect Immun. 1979 Sep;25(3):1066–1074. doi: 10.1128/iai.25.3.1066-1074.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  48. Yanagawa R., Honda E. Presence of pili in species of human and animal parasites and pathogens of the genuscorynebacterium. Infect Immun. 1976 Apr;13(4):1293–1295. doi: 10.1128/iai.13.4.1293-1295.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]

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