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. 2017 Jul 13;7:5343. doi: 10.1038/s41598-017-05557-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Binding isotherms and affinities of PMM/PGM(S108C) for phosphosugars derived from ¹⁵N TROSY NMR spectra. The binding isotherms were obtained using PCA implemented with TREND software^{16, 35}. PMM/PGM(S108C) was titrated with G1P (purple circles), G6P (blue squares), G16P (green triangles), or X1P (yellow-green diamonds). Fitted dissociation constants and Gibbs free energies are tabulated in the inset. The X1P titration of wt enzyme (gray pentagons) was collected at 298 K in the slow exchange regime and is adapted from ref. 16.