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. 2017 Jul 14;8:1269. doi: 10.3389/fmicb.2017.01269

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Copyright © 2017 Catón, Yurkov, Giesbers, Dijksterhuis and Ingham.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Overview of cryo-SEM image of developing TMB. (A) Trapping mycelia in a 180 μm diameter well after 2 days. (B) Growth out of the well after 3 days with limited dehydration prior to imaging, emphasizing the high degree of hydration. (C) As (B), but at a slightly more advanced stage and with more removal of water revealing hyphae and some phialides. (D) Mature TMB after 8 days showing most phialides at the apex (x). (E) As (D) but showing multiple TMB. (F) Phialides after 3 days. (G) Close-up of phialides at apex of the TMB after 8 days. Note the large spherical clusters containing large numbers of conidia. Scale bar indicates 40 μm for (A–C), 140 μm for (D), 60 μm for (E), and 5 μm for (F,G).