Table 1.

PCR primers used for identification, functional genes amplifications and nifH gene expression.

Gene	Primer	Sequence (5′ → 3′)	Product size	References
16S	pA-F	AGAGTTTGATCCTGGCTCAG	1,300–1,500 bp	Edwards et al., 1989
	pH-R	AAGGAGGTGATCCAGCCGCA
NifH	Pol-F	TGCGAYCC-SAARGCBGACTC	360 bp	Poly et al., 2001
	Pol-R	ATSGCCATCATYTCRCCGGA
PhCA	PhCA-F	TTGCCAAGCCTCGCTCCAAC	1,150 bp	Raaijmakers et al., 1997
	PhCA-R	CCGCGTTGTTCCTCGTTCAT
PRN	Prn-F	GGGGCGGGCCGTGGTGATGGA	786 bp	Souza and Raaijmakers, 2003
	Prn-R	YCCCGCSGCCTGYCTGGTCTG
HCN	HCN-F	ACTGCCAGGGGCGGATGTGC	587 bp	Ramette et al., 2003
	HCN-R	ACGATGTGCTCGGCGTAC
BOX	BOX A1R	CTACGGCAAGGCGACGCTGACG	50–5,000 bp	Rademaker and de Bruijn, 1997
ERIC	ERIC-1	ATGTAAGCTCCTGGGGATTCAC	50–5,000 bp
	ERIC-2	AAGTAAGTGACTGGGGTGAGCG
REP	REP-F	IIIICGICGICATCIGGC	50–5,000 bp
	REP-R	ICGICTTATCIGGCCTAC
Q-RT-PCR PRIMER
NifH	Pol-F	TGCGAYCC-SAARGCBGACTC		Poly et al., 2001
	Pol-R	ATSGCCATCATYTCRCCGGA
Glyceraldehyde 3-phosphate dehydrogenase	GAPDH-1	CTCTGCCCCAAGCAAAGATG		Niu et al., 2013
	GAPDH-2	TGTTGTGCAGCTAGCATTG