Figure 1.

Representative experiment of membrane wounding by LLO and repair kinetics in HeLa cells. HeLa cells were exposed to the indicated concentrations of LLO in M1 (+ 1.2 mM CaCl₂, solid lines) or M2 (without CaCl₂, dashed lines) containing 30 μM PI and incubated in the plate reader at 37°C for 30 min. Fluorescence intensities were measured every 5 min. The baseline fluorescence levels of control cells incubated without LLO, at each time point in M1 and in M2, were subtracted from the values obtained with cells incubated with LLO. Data are the average fluorescence intensities expressed in arbitrary unit ± standard deviations (S.D.) of triplicates for each experimental condition.