Figure 2.

LLO induces dose-dependent membrane wounding and repair in HeLa cells. HeLa cells were exposed to the indicated concentrations of LLO in M1 (solid lines) (A) or M2 (dashed lines) (B) supplemented with 30 μM PI and incubated in the plate reader for 30 min at 37°C. At each time point in M1 (A) and M2 (B), the baseline fluorescence level of control cells incubated without LLO were subtracted from the values obtained with cells incubated with LLO. Data are the average fluorescence intensities of four independent experiments, with the error bars representing the standard error of the mean (SEM). Statistical analyses correspond to the trend comparison (T, p < 0.0005) and the mean fluorescence intensities averaged across all time points (^*, p < 0.0005). Those analyses compared the different concentrations of LLO in M1 (A) or in M2 (B) and compared data obtained in M1 vs. M2 (M1/M2) at a given concentration of LLO (showed in the squared box). Statistical analyses are also recapitulated in Tables 1A,B.