Figure 4.

Effect of F-actin disassembly on plasma membrane repair. HeLa cells were pre-incubated in the presence of 5 μM cytochalasin D (CD, stock solution was stored in DMSO) for 10 min at 37°C, and the drug was maintained at the same concentration throughout the assay. Cells were exposed to 0.5 nM LLO, or not, in M1 or M2 supplemented with 30 μM PI. Control cells were incubated with a dilution of vehicle (DMSO) similar to cells treated with CD. Data are the average of four independent experiments, each performed in triplicate, and the error bars represent the standard error of mean (SEM). Statistical analyses correspond to the trend comparison (T, p < 0.0005) and the mean fluorescence intensities averaged across all time points (^*, p < 0.0005). Statistical analyses are also recapitulated in Tables 2A,B.