Table 3A.

Fluorescence intensity change over time (trend) was compared between LLO concentrations higher than 0.1 nM and that of 0.1 nM for M1 and M2 from a linear mixed effects model.

Trend analysis of fluorescence increase over time for C2C12 cells
	Trend estimate	p-values	95% CI of trend estimate
Dose effect	2.4171	<0.0001	1.3080	3.5262
M1 comparing LLO 2 to 0.1	0.1072	<0.0001	0.06825	0.1461
M1 comparing LLO 1 to 0.1	0.09803	<0.0001	0.05912	0.1369
M1 comparing LLO 0.5 to 0.1	0.09391	<0.0001	0.05501	0.1328
M1 comparing LLO 0.25 to 0.1	0.04633	0.0198	0.007420	0.08524
M2 comparing LLO 2 to 0.1	−0.01475	0.4559	−0.05366	0.02416
M2 comparing LLO 1 to 0.1	−0.01224	0.5360	−0.05115	0.02667
M2 comparing LLO 0.5 to 0.1	−0.00343	0.8624	−0.04234	0.03548
M2 comparing LLO 0.25 to 0.1	0.001384	0.9442	−0.03753	0.04029

Holm's procedure was used to adjust for multiple comparisons.