Table 5.

An example of a possible strategy for marker-assisted selection (MAS) within RILs of 541 × Ot1–3 intercross, considering QTL interaction

Objective of selection	QTL and alleles targeted by MAS in the chosen direction
Short, thick stem and long spike	PH3.3.B, PH5.4.A, PH3.5.B, PH5.1.B, PH6.1.B, ST2.1.B, ST5.4.A, SL5.2.A, (ST1.1–SL1.3)A, (ST4.2–SL4.2)A, (PH4.2–SL4.3)A, (PH7.1–ST7.1-SL7.1.)A, (PH7.2–ST7.2.)A
+ high thousand-kernel weight	TKW1.1.A, TKW1.6.A, TKW3.2.A, TKW3.3.A, TKW4.3.A, TKW4.4.A, TKW4.5.A, TKW6.2.A, TKW7.2.A
+ early heading date	HD1.2.A, HD2.1.A, HD5.3.B, HD5.8.A, HD5.9.A, HD5.10.A, HD5.11.A, HD6.1.A, HD7.4.B

A = An allele introduced by the 541 parental line; B = an allele introduced by the Ot1–3 parental line